Scientific publications

Title: Successful three-year outcome in a patient with allogenous sternal bone graft in the treatment of massive post-sternotomy defects

Author: Martin Kaláb a, Jan Karkoška b, Milan Kamínek c, Petr Šantavý a
Department of Cardiosurgery, University Hospital Olomouc and Faculty of Medicine, Palacky University Olomouc, I.P. Pavlova 6, Olomouc 775 20, Czech Republic
National Cell and Tissue Centre, Palachovo náměstí 726/2, Brno, CzechRepublic
Department of Nuclear Medicine,University Hospital Olomouc and Faculty of Medicine, Palacky University Olomouc,  I.P.Pavlova 6, Olomouc 77520, Czech Republic

Source: International Journal of Surgery Case Reports 7 (2015) 6-9
Abstract: Deep sternal wound infection is a life-threatening complication of longitudinal median sternotomy with extensive loss of sternal bone tissue and adjacent ribs. Wound dehiscence cases with no loss of bone tissue can be resolved via osteosynthesis using titanium plates. Unfortunately, this cannot be used in cases of massive bone tissue loss defects due to insufficient support for fixing the plate material caused by missing bone surface which increasing the risk of osteosynthesis failure. We describe the treatment outcome of sternal dehiscence with massive bone tissue loss defects using an allogenous sternal bone graft.

Publication available here

Title: Mutation Frequency Dynamics in HPRT Locus in Culture-Adapted Human Embryonic Stem Cells and Induced Pluripotent Stem Cells Correspond to Their Differentiated Counterparts.

Author: Krutá M1, Seneklová M, Raška J, Salykin A, Zerzánková L, Pešl M, Bártová E, Franek M, Baumeisterová A, Košková S, Neelsen KJ, Hampl A, Dvořák P, Rotrekl V.
Stem Cells Development, 2014 Jul 25
The genomic destabilization associated with the adaptation of human embryonic stem cells (hESCs) to culture conditions or the reprogramming of induced pluripotent stem cells (iPSCs) increases the risk of tumorigenesis upon the clinical use of these cells and decreases their value as a model for cell biology studies. Base excision repair (BER), a major genomic integrity maintenance mechanism, has been shown to fail during hESC adaptation. Here, we show that the increase in the mutation frequency (MF) caused by the inhibition of BER was similar to that caused by the hESC adaptation process. The increase in MF reflected the failure of DNA maintenance mechanisms and the subsequent increase in MF rather than being due solely to the accumulation of mutants over a prolonged period, as was previously suggested. The increase in the ionizing-radiation-induced MF in adapted hESCs exceeded the induced MF in nonadapted hESCs and differentiated cells. Unlike hESCs, the overall DNA maintenance in iPSCs, which was reflected by the MF, was similar to that in differentiated cells regardless of the time spent in culture and despite the upregulation of several genes responsible for genome maintenance during the reprogramming process. Taken together, our results suggest that the changes in BER activity during the long-term cultivation of hESCs increase the mutagenic burden, whereas neither reprogramming nor long-term propagation in culture changes the MF in iPSCs.

Available in pdf.

Title: Injectable Hydrogel Functionalised with Thrombocyte - Rich Solution and   Microparticles for Accelerated Cartilage Regeneration

Author: M. Rampichová, M. Buzgo, B. Křížková, E. Prosecká, M. Pouzar, L. Štrajtová
Abstract: Articular cartilage defects arise due to injury or osteochondral disease such  as osteonecrosis or osteochondritis dissecans. In adult patients cartilage  has minimal ability to repair itself and the lesions develop into degenerative  arthritis. Overcoming the low regenerative capacity of the cartilage cells and  the Hayflick limit poses a challenge for the therapy of osteochondral defects.  Composite scaffolds with appropriate biomechanical properties combined with a  suitable blend of proliferation and differentiation factors could be a  solution. The aim of this in vitro study was to develop a novel functionalised  hydrogel with an integrated drug delivery system stimulating articular  cartilage regeneration.

Available in pdf.

Title: Use of allogenous bone graft and osteosynthetic stabilization in treatment of massive post-sternotomy defects

Author: Martin Kaláb, Martin Molitorb, Barbara Kubešová, Vladimír Lonskya
Source: Oxford Jounals - European Journal of Cardio-Thoracic Surgery
Abstract: Thoracic stabilization using transverse plate fixation represents a modern and safe method of sternal dehiscence treatment. However, it still remains difficult to apply in cases of massive loss of bone tissue of the chest wall. An unsatisfactory stability of thorax often results in severe respiratory insufficiency, and also affects healing of soft tissue closure while increasing the risk of development of chronic fistulas and other dehiscences. In the reported case, we opted for a unique treatment of massive post-sternotomy defect using an allogenous bone graft of calva. Transverse titanium plates were applied to achieve stabilization of bone grafts and chest wall.

Available in pdf.  

Title: Production of Clinical Grade Mesenchymal Stromal Cells

Autor: Pytlík, Slanař, Stehlík and Matějkova
Source: InTech
Publication: Regenerative Medicine and Tissue Engineering - Cells and Biomaterials,  ISBN: 978-953-307-663-8

Available in pdf.